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| Descripción | HiFluoro Pseudomonas HiVeg Agar Base Technical Data | |
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HiFluoro Pseudomonas HiVeg Agar Base
MV1469
HiFluoro Pseudomonas HiVeg Agar Base is recommended for selective isolation of Pseudomonas aeruginosa from
clinical and non-clinical specimens by fluorogenic method.
Composition**
Ingredients
HiVeg Peptone No.2
Magnesium chloride
Potassium sulphate
Cetrimide
Fluorogenic mixture
Agar
Final pH ( at 25°C)
**Formula adjusted, standardized to suit performance parameters
Gms / Litre
18.000
1.400
10.000
0.300
2.050
15.000
7.2±0.2
Directions
Suspend 46.75 grams in 1000 ml distilled water containing 10ml glycerol. Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Pseudomonas aeruginosa (also known as Pseudomonas pyocyanea ) is a gram-negative, aerobic, rod-shaped bacterium.
Like other Pseudomonads, P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein
(yellow-green and fluorescent), and pyorubin (red-brown). King et al developed Pseudomonas Agar P (i.e. King A media)
for enhancing pyocyanin and pyorubin production and Pseudomonas Agar F (i.e. King B media) for enhancing fluorescein
production (1). HiFluoro Pseudomonas HiVeg Agar Base is prepared by completely replacing animal based peptones with veg
peptones. This media is a modification of HiFluoro Pseudomonas Agar Base which is devised based on the formula described
by King et al. (1) except fluorogenic mixture. It is used as the selective medium for the isolation of P. aeruginosa from
pus, sputum and drains etc.
Cetrimide (Cetyltrimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than P. aeruginosa
. It acts as a quaternary ammonium compound, cationic detergent that causes nitrogen and phosphorus to be released from
bacterial cells other than P.aeruginosa . P.aeruginosa cleaves the fluorogenic compound to release the fluorogen which
produces a visible fluorescence under long wave UV light.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber colourec opalescent gel forms in Petri plates
Reaction
Reaction of 4.675 % w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
Please refer disclaimer Overleaf.
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